Fig. 7

Human breast cancer cell p21 expression is increased over time in co-cultures with EO cells. MDA-MB-231, MDA-MB-231BRMS, or MCF-7 cells were co-cultured with either MC3T3-E1 osteoblasts or EO cells in 35 × 10 mm dishes. 6, 10, 14, or 18 days later, lysates were obtained and examined for human breast cancer cell expression of human-specific p21 by western blot. N = 3 dishes per condition, per time point. a MDA-MB-231 cells co-cultured with either MC3T3-E1 or EO-231 cells, b MDA-MB-231BRMS cells co-cultured with either MC3T3-E1 or EO-BRMS cells, c MCF-7 cells co-cultured with either MC3T3-E1 or EO-MCF7 cells