Fig. 6

MEDI3039 inhibited established lung metastases and extended animal survival in the MB231T lung metastasis model. a Survival curve of mice treated with MEDI3039 (0.3 mg/kg) or vehicle. p value, HR, and 95% of CI were obtained with log-rank (Mantel–Cox) test, compared with the vehicle control group. The lung from one of two sentinel mice sacrificed is shown in the inset above the graph demonstrating grossly visible established lung metastases at day 21. b Mice lung tissue from both vehicle- and MEDI3039-treated groups, fixed with Bouins’ solution. c Quantitative analysis of metastasized tumors in the lung. The number of tumors was classified according to their size. Data is presented as mean ± SEM, and Student’s t test was used for the statistical analysis. The red bars represent MEDI3039-treated mice that were sacrificed prior to the end of the experiment. The green bars represent data from 4 mice still alive at the end of the experiment. d Two representative H&E-stained lung samples per vehicle- or MEDI3039-treated group. Top: H&E-stained lung tissue. Bottom: red-colored area indicates tumor region, green-colored area indicates non-cancerous region. Areas circled with light dotted green line indicate the diaphragm and lymph nodes excluded from analysis. e Quantitative analysis of microscopic tumors in the lung. Data is presented as median area of lung involved with tumor with IQR. Note that all mice in vehicle groups were dead or euthanized on day 72 or 73, while all 5 mice in MEDI3039 were alive and euthanized at day 90