Fig. 2
From: HER2 regulates HIF-2α and drives an increased hypoxic response in breast cancer
Increased upregulation of known hypoxia response genes in MCF7-HER2. Western blotting (a) and bar charts showing mean densitometry values (error bars = SEM) (b) demonstrating the increased upregulation of HIF-2α (P = 0.028 (n = 4)) and CAIX (P = 0.033 (n = 5), two-way ANOVA) in the MCF7-HER2 cell line after 24, 48 and 72 h hypoxia when compared to wild-type MCF7. No significant difference was observed for HIF-1α. c Gene expression data from an Illumina BeadChip Array was used to compare the expression of known HIF target genes. Four genes reported to be specific for each of HIF-1 and HIF-2 are shown alongside four genes targeted by both factors. For each gene, mean expression is shown for MCF7 and MCF7-HER2 cell lines cultured in normoxia, acute (24 h) hypoxia or chronic (> 10 weeks) hypoxia (error bars = SEM). This demonstrates the general trend toward and increased hypoxic regulation of these genes in the HER2-overexpressing cell line