Fig. 3

MDMX and MDM2 provoke in vitro MDA-MB-231 cell migration without altering cell proliferation. a Representative Western blot demonstrating the levels of MDM2, MDMX, and mtp53 in 231.mir30.vector, 231.shmdm2, and 231.shmdmx cells (lanes 1, 2, and 3, respectively). Fifty micrograms of lysate was loaded per lane. Actin was used as a loading control. b The number of cells was determined by hemocytometer cell counting. Cells (n = 50,000) were seeded in triplicate, and cell counting was performed at 2, 4, 5, and 6 days. Dots represent mean values, and error bars represent SD. Experiments were carried out with three biological replicates. c Wound closure was observed by phase-contrast microscopy and photographed at 0 and 12 h. One representative image from each group at 0 and 12 h is shown. d The wound area was measured by using NIS-Elements software (Nikon Instruments, Melville, NY, USA). The percentage of wound closure was quantified from four independent biological experiments. The p value was obtained by two-tailed unpaired t test