Fig. 2

Establishment of gene-introduced mammary glands. a A representative image showing luciferase activity of primary outgrowths at day 56 after mammary epithelial cell injection into cleared fat pad. b Microscopic view of resected fat pads from a. Red fluorescence (middle panel) shows mCherry marker expression. Right panel shows carmine staining to show whole mammary glands in this fat pad. Scale bar = 1 mm. c IVIS images of secondary outgrowth at day 75 after transplantation of mCherry-expressing fragments of one primarily reconstituted mammary gland. Right panels are microscopic images of the dissected mammary gland from the site indicated by the yellow dotted boxes in the left images. Lower panels show mCherry marker expression. Scale bars = 5 mm