Fig. 2From: CD68, CD163, and matrix metalloproteinase 9 (MMP-9) co-localization in breast tumor microenvironment predicts survival differently in ER-positive and -negative cancersDetection of CD68, CD163, and matrix metalloproteinase 9 (MMP-9) using multiplex quantitative immunofluorescence (QIF) in breast cancer. Representative fluorescence images showing the detection of tumor-associated macrophage (TAM) subsets in breast cancer samples by simultaneous staining of DAPI (blue channel), cytokeratin (fluorescein isothiocyanate, green channel), CD68 (Cy5, red channel), CD163 (Cy3 Plus, yellow channel), and MMP-9 (Cy7, magenta channel). The insert shows higher magnification of stromal TAMs. Bar = 100 μmBack to article page