Fig. 1

miR-140-3p-1 is lost during breast cancer progression. a Sequences of mature miR-140-3p-1 and miR-140-3p-2 isoforms. b qPCR showing miR-140-3p-1 and miR-140-3p-2 expression in a MCF10A-based breast cancer progression model. miRNA levels were measured by TaqMan-based qPCR probes. Fold change calculated relative to the cell line with the lowest miRNA expression (highest cycle threshold (Ct)), which was set as 1. Differential miRNA expression for the rest of the comparisons was determined by calculating the fold change of miRNA above this lowest expression level using the Pffafl differential Ct method. Values are also normalized to small nucleolar RNA (RNU44) and represent mean fold change ± SEM