Fig. 6

CT + shear stress (SS) demonstrate upregulation of reactive oxygen species (ROS)-responsive and nitric oxide (NO)-responsive genes. a Transcriptional profile of the selected ROS-responsive genes (Sod1, Cat, Nox1, Nox4, and Gpx1; i). (ii) Flow cytometry analysis of ROS generation of breast cancer cells derived from chemotherapy-treated patients (CT-PCs) treated with N-acetylcysteine (NAC) on day 3, 5, 7, and 10 during +SS (p value compared with adherent cells). iii, The number of suspended cells was counted on day 3, 5, 7, and 10 in the presence or absence of NAC treatment. b Transcriptional profile of the selected NO-responsive genes (Nos1, Nos2, and Noxtrin; (i)). Intracellular NO analysis was performed on CT-PC and CT + SS with 1 mM N(G)-nitro-l-arginine-methyl ester (L-NAME) by measuring the fluorescence NO probe (ii). The number of suspended cells was counted on day 3, 5, 7, and 10 in the presence or absence of L-NAME (iii). The data presented here are presented as mean ± SEM and are representative of three independent experiments. Statistically significant differences were tested at p < 0.05