Fig. 2

ED and EAD induce growth arrest. a qRT-PCR of few genes, related to cell growth arrest and death (identified by array analysis), in MDA-MB-231 cells treated with entinostat (2.5 μM), ATRA (1 μM), and doxorubicin (0.2 μM) singly, and combinations, for 48 h. b Western blot analysis of cyclin A and cyclin D1 on MDA-MB-231 cells, treated as described in text. Flow cytometry determination of percentage of cell cycle distribution of (c) MDA-MB-231 and (d) SUM-149 cells treated with different groups containing doxorubicin 6.25–200 nM (doxorubicin 12.5 nM highlighted on right), for 48 h. RPL39 and GAPDH used as controls for qRT-PCR and western blot, respectively. Student’s t test performed, mean (± SEM) of triplicate results shown. *Compared to entinostat in qRT-PCR and doxorubicinin flow cytometry; #compared to entinostat in flow cytometry: *p < 0.05, **/##p < 0.01, ***p < 0.001. E entinostat, A all-trans retinoic acid, D doxorubicin (Dox), Veh vehicle, GAPDH glyceraldehyde 3-phosphate dehydrogenase