Fig. 1

Detection of mucosal associated invariant T cells from human breast ducts. a Left plots: Staining for Vα7.2 T-cell receptor TCR expression by T cells from peripheral blood (peripheral blood mononuclear cells [PBMCs]) or human breast ducts. Right plot: Quantification of Vα7.2⁺ T-cell frequencies in PBMCs vs. breast duct tissue. PBMCs and breast tissue samples were not obtained from the same donors. b Left plots: Costaining of CD3⁺ cells from blood vs. breast ducts by anti-Vα7.2 antibody and 5-(2-oxoprophylideneamino)-6-d-ribitylaminouracil (5RU)-loaded MR1 tetramer. Right plot: Quantification of the fraction of the Vα7.2⁺ T cells costained by the MR1-5RU tetramer for PBMCs vs. breast duct cells. c Flow cytometric analysis of CD161, interleukin (IL)-18Rα, and promyelocytic leukemia zinc finger protein (PLZF) staining for the subset of breast duct T cells that costain for Vα7.2 and MR1-5RU tetramer (black line histogram) compared with Vα7.2-negative T cells from the breast (gray filled histogram). n.s. Not significant