Fig. 3

ETV4 enhances proliferation, migration, invasion, and anchorage-independent growth capacity of MMT mammary cancer cells. a MMT-Ctrl and MMT-ETV4 cell proliferation analysis by cell counting. The two charts represent the number of counted cells at 10, 35, 55, 80, and 100 hours. Experiments were conducted three times in triplicate. Error bars indicate SD. ***P ≤ 0.001; **P ≤ 0.01; *P ≤ 0.1. b MMT-Ctrl and MMT-ETV4 cell migration analysis using a Boyden chamber culture system. Histograms represent the relative number of counted cells that migrated to the lower side. The number of MMT-Ctrl cells was arbitrarily = 1. Experiments were conducted three times in triplicate. Error bars indicate SD. ****P ≤ 0.0001. The lower panel depicts a representative picture of each experiment. Scale bar = 100 μm. c MMT-Ctrl and MMT-ETV4 cell invasion analysis using a Boyden chamber culture system coated with Matrigel®. Histogram represents the relative number of cells that invaded to the lower side. The number of MMT-Ctrl cells was arbitrarily = 1. Experiments were conducted three times in triplicate. Error bars indicate SD. ****P ≤ 0.0001. The lower panel depicts a representative picture of each experiment. Scale bar = 100 μm. d Anchorage-independent growth. MMT-Ctrl and MMT-ETV4 cells were cultured for 10 days in soft agar. This histogram represents the number of clones counted for experimental time point. Soft agar assays were conducted three times in triplicate. Magnification × 5. Error bars indicate SD. ****P ≤ 0.0001. The lower panel depicts a representative picture of each experiment. Scale bar = 100 μm