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Fig. 3 | Breast Cancer Research

Fig. 3

From: Correction to: Nongenomic oestrogen signalling in oestrogen receptor negative breast cancer cells: a role for the angiotensin II receptor AT1

Fig. 3

Effect of GPCR antagonism on 17β-oestradiol and EGF induced cell proliferation, raf phosporylation and cAMP production in breast cancer cells. (b) SKBR3 and MCF-7 cells were pre-treated with the GPCR antagonist pertussis toxin (50 ng/ml) for 1 h before 10 min of incubation with EGF (10 ng/ml) and 17β-oestradiol (10− 8 mol/l) alone and in combination. 40 μg protein was electrophoresed on a 10% gel and transferred to nitrocellulose. The membrane was probed with rabbit anti-Phospho-Raf antibody (Ser259 - Cell Signalling # 9421) and mouse anti-Raf antibody (Santa Cruz sc-373,722)

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Breast Cancer Research

ISSN: 1465-542X

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