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Fig. 5 | Breast Cancer Research

Fig. 5

From: Glucocorticoids promote transition of ductal carcinoma in situ to invasive ductal carcinoma by inducing myoepithelial cell apoptosis

Fig. 5

Immunohistochemical and immunofluorescence images of human samples from patients with ductal carcinoma in situ (DCIS) and DCIS + invasive ductal carcinoma (IDC). a CD10 (myoepithelial cells) immunohistochemical analysis of samples from patients. Scale bar = 100 μm. b Double immunofluorescence of p63 (myoepithelial cells) and cleaved caspase 3 and Hoechst dye as a nuclei counterstain. Upper panel: patients with DCIS; bottom panel: patients with DCIS + IDC. White scale bar = 20 μm and red scale bar = 50 μm. c Quantification of apoptotic myoepithelial cells in DCIS (13 patients) and DCIS + IDC (15 patients). The Mann-Whitney test was used for statistical analysis

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