Fig. 4
In vivo chicken embryo chorioallantoic membrane (CAM) system for growing MCF10DCIS tumours. a Timeline of CAM model indicating how the inoculation of MCF10DCIS cells was performed and how the tumours were treated. b Representative tumour images after treating with control, cortisol 0.7 μM, RU486 0.5 μM and cortisol + RU486 for 5 days. c Graphical representation of tumour volume in mm3. d α-Smooth muscle actin (α-SMA) (in red) immunofluorescence of the tumours after treatment with cortisol 0.7 μM, RU486 0.5 μM and cortisol + RU486. Hoechst dye was used to counterstain nuclei. Scale bar =20 μm. e Quantification of acini size (μm). Bottom panel: representation of percentage of α-SMA-positive area in tumours. Scale bar = 50 μM. f Images of double p63 (myoepithelial cells) and cleaved caspase 3 immunofluorescence in tumours. Scale bar = 50 μM. g Graphical representation of apoptotic myoepithelial cells quantification in tumours under the different treatments. One-way analysis of variance was used for statistical analysis followed by Tukey post-hoc test