Fig. 5

Net1 deletion inhibits PyMT-dependent ERK1/2 and Akt1 activation. a Schematic depicting PyMT-initiated signaling. b Representative analysis of Src, ERK1/2, and Akt1 phosphorylation on their activating sites in whole tumor lysates. Each lane represents a tumor from a distinct animal. c Quantification of western blotting in whole tumor lysates. Three to 6 tumors per genotype examined. d Western blot analysis of proteins coimmunoprecipitating with PyMT from tumors of genotypes shown. Representative experiment from four independent experiments. Errors are standard error of the mean. *P < 0.05; **P < 0.01. AKT1 v-akt murine thymoma viral oncogene homolog 1, ERK1 extracellular signal regulated kinase 1, Net1 neuroepithelial transforming gene 1, p85 phosphatidylinositol 3-kinase regulatory subunit p85α, PIK3CA phosphatidylinositol 3-kinase catalytic subunit A, PP2A protein phosphatase 2A, PyMT polyoma middle T antigen, Shc Src homology domain 2 containing, Src SRC proto-oncogene, non-receptor tyrosine kinase