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Fig. 6 | Breast Cancer Research

Fig. 6

From: Isocitrate dehydrogenase 1–snail axis dysfunction significantly correlates with breast cancer prognosis and regulates cell invasion ability

Fig. 6

Isocitrate dehydrogenase 1 (IDH1) knockdown promoted breast cancer invasion through hypoxia-inducible factor-1 alpha (HIF-1α) and NFkB signaling. a and b Western blot analysis of IDH1, HIF-1α, NFkB, snail, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) are shown in MDA-MB-231 cells transfected with si-IDH1 and scramble control (N.C). c and d Schema of the luciferase reporter constructs (upper panels). The relative luciferase activity of HIF-1α and NFkB promoters were determined in breast cancer cells transfected with si-IDH1 and scramble control. Firefly luciferase activity was used as the normalization control. DMSO, dimethyl sulfoxide

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