Fig. 1

Isocitrate dehydrogenase 1 (IDH1) expression was significantly lower in breast cancer a Protein levels of IDH1 and IDH2 in breast cancer were examined and matched through western blotting with those in adjacent normal tissues obtained from 10 patients. b Expression levels of IDH1 and IDH2 were further quantified using Image J. N, normal; T, tumor. c Expression levels of IDH1 were assessed in breast cancer tissues (n = 51) and adjacent normal tissues (n = 29) using a real-time PCR approach. CT, cycle threshold. d Expression levels of IDH1 transcripts were analyzed in breast cancer tissues (n = 1102) and compared with those in adjacent normal tissues (n = 113) from The Cancer Genome Atlas database. e Immunohistochemistry (IHC) analysis of IDH1 expression in the breast cancer tissue microarray from 309 patients. Representative photomicrographs are presented. Representative photomicrographs showed negative (−), weak (+), moderate (++), and strong (+++) staining in invasive ductal carcinoma (IDC) tissues. f, g IDH1 levels were significantly lower in ductal carcinoma in situ and IDC tissues compared with adjacent normal tissues