Fig. 5
From: Integrin-uPAR signaling leads to FRA-1 phosphorylation and enhanced breast cancer invasion
Vitronectin-induced breast cancer invasion requires urokinase/plasminogen activator urokinase receptor (uPAR) and Fos-related antigen 1 (FRA-1) expression. BRC-31 (a) or HCC1143 (b) breast cancer cells were plated in Boyden chambers in which the bottom surface was coated with vitronectin and the upper surface of the chamber coated with Matrigel mixed with vitronectin. Cells were allowed to invade towards serum-free medium for 24 hours. Quantification of breast cancer cell invasion and representative images are shown. Data from nine independent experiments plotted with the error bars representing the standard deviation. P values are as indicated. Immunoblot analyses of protein lysates from BRC-31 (c) or HCC1143 (d) cells transfected with scrambled (Scr), fosl1 (fosl1 knockdown (KD)) or plaur (plaur KD) siRNAs. α-Tubulin served as a loading control and representative blots from one of nine independent sets of lysates are shown