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Fig. 8 | Breast Cancer Research

Fig. 8

From: FUT8 promotes breast cancer cell invasiveness by remodeling TGF-β receptor core fucosylation

Fig. 8

Pharmacological fucosyltransferase (FUT) inhibition reduces invasive ability and lung metastasis of breast carcinoma cells. a Core fucosylation was suppressed by treatment with a FUT inhibitor, 2-fluorinated-peracetyl-fucose. MDA-MB-231 cells were treated with 400 μM 2-fluorinated-peracetyl-fucose for 5 days. Core fucosylation of cells was examined by fluorescence-activated cell sorting (FACS) with Lens culinaris lectin (LCA) binding assay. b Cell invasion measured by the Boyden-chamber Transwell assay with Matrigel coating. Data are mean ± SD. **P < 0.01. c FUT inhibitor treatment did not affect xenograft tumor growth of 4T1 cells in vivo. Tumor volumes were measured weekly in nude mice mammary fat pads injected with 4T1 cells. Data are mean ± SD (n = 8 in each group). d FUT inhibitor treatment inhibited the lung metastasis of the 4T1 tumor model. Number of lung metastatic nodules in nude mice with mammary fat pad injection of 4T1 cells was counted and displayed by box-whisker plot (left). Data are median (horizontal line), upper and lower quartiles (box edges) and ranges (whiskers) (n = 8 in each group). **P < 0.01 compared with control. Representative images of H&E-stained lung sections from each group are shown (right). Arrowheads mark metastatic nodules in the lung. Scale bar = 200 μm

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