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Fig. 7 | Breast Cancer Research

Fig. 7

From: Epithelial requirement for in vitro proliferation and xenograft growth and metastasis of MDA-MB-468 human breast cancer cells: oncogenic rather than tumor-suppressive role of E-cadherin

Fig. 7

a Epithelial-mesenchymal plasticity status in vivo as xenografts in mice. E-cadherin was assessed by double-immunohistochemistry (IHC) of E-cadherin (brown) and vimentin (red). Vimentin-positive cells at tumor-stroma interface are indicated by blue arrowheads, and at tumor-necrosis border they are indicated by red arrowheads. Red-channel images are shown for vimentin IHC clarity. All scale bars = 50 μm. b Enlargement of 468-pcDNA3 and 468-CDH1 tumors showing differences in vimentin-E-cadherin staining. c Quantitative polymerase chain reaction expression of the same modified cells grown in 2D versus 3D. ΔCT Cycle threshold change, SCR Scrambled, WT Wild type

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