Fig. 6From: Epithelial requirement for in vitro proliferation and xenograft growth and metastasis of MDA-MB-468 human breast cancer cells: oncogenic rather than tumor-suppressive role of E-cadherin a Immunohistochemistry for CD31 (left panel), with smaller vessels indicated by red arrowheads and larger vessels indicated by green arrowheads. b Plot of the average number of CD31-stained blood vessels in five random microscopic fields (original magnification × 200) per tumor. Statistical significance was assessed by one-way analysis of variance (no difference across groups). c. Quantitative polymerase chain reaction data for hypoxic indicator gene carbonic anhydrase 9 (CAIX) (n = 3). Error bars represent SE. ΔCT Cycle threshold change, SCR Scrambled, WT Wild typeBack to article page