Fig. 2

AKT1^low quiescent cancer cells (QCCs) are found in primary breast tumor tissue using quantitative immunofluorescence microscopy. a White arrow points to an example of a QCC cluster from control tumor 3, section 17 by confocal microscopy at × 60; upper panel shows merged image and lower panel shows single color images. b Upper panel shows the same QCC cluster as in a (red arrow) as × 20 composite image (pan-AKT (green), H3K9me2 (yellow), and HES1 (red)). In the lower panel, the area shown in the × 20 image is represented as a digital tumor map identifying the same QCCs (red arrow) shown in a with high fidelity. c An example of a whole-section digital tumor map for tumor 3, section 17, where QCCs are shown by red dots and other cancer cells by blue dots. Image coordinates are listed on each axis. Red arrow points to the same QCC cluster shown in a and b