Fig. 5

Depletion of cyclin D1 and p21 prevents mammary tumor growth and local invasion. a Parental SCP2 and p21/cyclin D1 double knockdown SCP2 cells were implanted into the mammary fat pad of four- to six-week-old female Balb/c nude mice. Mammary tumor growth was measured from two sets of mice and quantified for the tumor size at the indicated times (six per group; error bars indicate SEM). b Representative photographs show hematoxylin and eosin staining of the mammary gland (tumor and surrounding tissues) of mice at eight weeks post-injection. c Representative photographs show PTGS2 staining of parental (top panel) and p21/cyclin D1-depleted (lower panel) mammary tumors at eight weeks post-injection. Two mice were used in each group. d Representative radiographs of skeletal lesions in two group mice (parental and p21/cyclin D1-depleted SCP2) were taken by X-ray using Faxitron. Parental and p21/cyclin D1-depleted SCP2 cells were injected in tibia. The lesions are highlighted by arrows