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Fig. 7 | Breast Cancer Research

Fig. 7

From: Imaging the mammary gland and mammary tumours in 3D: optical tissue clearing and immunofluorescence methods

Fig. 7

Imaging of see deep brain (SeeDB)-cleared mammary tumours using 2-photon excitation microscopy. Two-photon imaging of SeeDB-cleared human epidermal growth factor receptor 2 (HER2)-positive mammary tumours immunostained with HER2 (green) and 4′,6-diamidino-2-phenylindole (DAPI) nuclear staining (blue) and magnified view (inset). Orthagonal views show XZ (purple line and box) and YZ (red line and box) planes. Thin optical slices (2 μm) and their depth (z value) relative to the first image in the image sequence are also shown. See additional file 18 for a high resolution version of these PDFs and http://rdcu.be/lT3Z for additional high resolution examples of these imaging techniques [38]

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Breast Cancer Research

ISSN: 1465-542X

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