Fig. 6

Three-dimensional confocal imaging of mouse mammary tumours cleared with the passive clarity technique (PACT)-sorbitol refractive index matching solution (sRIMS), clear unobstructed brain imaging cocktails (CUBIC) and see deep brain (SeeDB) methods. PACT-sRIMS cleared tumour tissue (a), CUBIC-cleared tumour tissue (b) and SeeDB-cleared tumour tissue (c). Images show maximum intensity projections of 4′,6-diamidino-2-phenylindole (DAPI) nuclear staining and human epidermal growth factor receptor 2 (HER2) and K8 immunostaining, with thin optical slices (1 μm) and their depth (z value) relative to the first image in the image sequence. These images are representative of at least two regions acquired. See additional file 18 for a high resolution version of these PDFs and http://rdcu.be/lT3Z for additional high resolution examples of these imaging techniques [38]