Fig. 5

Activated nuclear factor kB (NF-kB) in invasive lobular carcinoma endocrine-resistant long-term estrogen-deprived (ILC-LTED) cells drives WNT4 expression. a Transcription factor reporter arrays were performed as described in the Methods section. Data are expressed as relative luciferase units (RLU) normalized to RLU from a negative control (luciferase without a response element). *p < 0.05; n.s., not significant (t test). Statistical tests were not corrected for multiple comparisons in this experiment, owing to the hypothesis-generating nature of this semibiased screen. b Breast cancer cell lines (BCCLs) were maintained in their respective standard conditions. Immunoblotting was performed as described in the Methods section. c BCCLs were reverse transfected with 10 nM small interfering (siRNA), and RNA was collected 60 h posttransfection. Bars represent the mean of biological triplicate ± SD. *p < 0.05 by analysis of variance of expression vs siSCR (Dunnett’s multiple comparisons test). d and e BCCLs were reverse-transfected with 10 nM siRNA, and lysates and/or RNA were collected 48 h posttransfection. d Immunoblotting was performed as described in the Methods section. Images are representative of duplicate experiments. e Gene expression data are shown as means of biological triplicate ± SD. p = not significant for siSCR vs siWNT4 (t test). ER Estrogen receptor, 44PE SUM44PE cell line, MM134 MDA-MB-134-VI