Fig. 4

WNT4 dependence in invasive lobular carcinoma endocrine-resistant long-term estrogen-deprived cells (ILC-LTED) cells is linked to endocrine response context. a Breast cancer cell lines were reverse-transfected with increasing concentrations of siWNT4 or 12.5 nM siSCR, or were mock-transfected (Ctrl). Growth was assessed 6 days posttransfection. siSCR was toxic in 134:L/A and 44:L/B, and these lines were not included in analyses or future small interfering RNA (siRNA) experiments. b Cells were reverse transfected with 35 nM siRNA and treated with increasing concentrations of ICI 182,780 (fulvestrant; ICI) approximately 16 h posttransfection. Growth was assessed 6 days posttreatment. *p < 0.05; bottom of nonlinear regression for siSCR vs siWNT4. 44PE SUM44PE cell line, MM134 MDA-MB-134-VI