Fig. 3

Expression and regulation of WNT4 in invasive lobular carcinoma endocrine-resistant long-term estrogen-deprived (ILC-LTED) cells correlates with use of the WNT4 estrogen receptor binding site (ERBS). a Breast cancer cell lines (BCCLs) in their respective standard conditions were treated in biological triplicate with 0.1 % EtOH or 1 μM ICI 182,780 (fulvestrant; ICI). RNA was harvested 24 h posttreatment. Bars represent mean ± SD. *p < 0.05 for vehicle control vs ICI (t test). b BCCLs were treated as in (a) for 60 minutes. Chromatin immunoprecipitation (ChIP) was performed as described in the Methods section. Data derived from single experiment, but are representative of duplicate experiments. “E2 status” denotes the hormone status of the experimental culture medium. ⁺FBS-containing medium; ⁻Charcoal-stripped FBS-containing medium. qPCR Quantitative polymerase chain reaction, E2 17β-Estradiol, MM134 MDA-MB-134-VI, SUM44 SUM44PE cell line