Fig. 5

Variance of TILs scores in breast cancer. a The variance for each marker within cores of the same tumor are expressed as intraclass correlation coefficients (ICC). ICC was of 0.411 for CD3, 0.324 for CD8, and 0.252 for CD20. b The analysis included the marker change between FOVs in the same tumor section (blue), between serial sections of the same core (orange) and between cores of the same tumor (green). Variance components of TILs scores from the same cancer indicate that 66–69 % of the variance is attributable to signal differences between fields of view of the same section, 30–33 % is due to differences between biopsies from different areas of the cancer and <2 % is due to differences between serial cuts from the same biopsy