Fig. 2
From: Neutrophils drive accelerated tumor progression in the collagen-dense mammary tumor microenvironment
Lymphocyte recruitment is not altered in late-stage collagen-dense tumors. a Flow cytometry gates used to determine lymphocytes. b Percentage of CD45+ immune cells in wild-type (WT) and collagen-dense (COL) mammary tumors (n = 6). c Flow cytometry of CD45+ cells comparing CD3 to CD11b in WT (left) and COL (right) mammary tumors, and the percentage of CD45+CD3+ T-cells present (n = 7) (d), CD45+CD3+CD8+ cytotoxic T-cells (e and f), CD45+CD3+CD4+ helper T-cells (e and g), and CD45+CD3+CD4+CD25+Foxp3+ regulatory T cells (h and i) (n = 6). Purple number (lower right) represents the number of total events in each graph. These cells were fixed for intracellular staining. FSC forward scatter, SSC side scatter, PE phycoerythrin