Table 2 Antibodies, dilutions and methods of evaluation
Target | Manufacturer | Catalog number (type of staining) | Dilution | Incubation time | Control tissue | Method of evaluation |
|---|---|---|---|---|---|---|
PD-1 | Novus | NBP1-88104 (cytoplasmic) | 1:100 | 30’ min | LN | SQ |
PD-L1 | AbD Serotec | AHP2128 (membranous/cytoplasmic) | 1:50 | 40’ min | HSM | SQ |
PD-L2 | R&D systems | AF1224 (membranous/cytoplasmic) | 1:500 | 30’ min | LN | SQ |
CTLA4 | Santa Cruz | SC-376016 (cytoplasmic) | 1:50 | 40’ min | LN | SQ |
GFAP | Dako | M0761 (cytoplasmic) | RU | 30’ min | B | SQ |
CD4 | Dako | IR649 (membranous) | RU | 20’ min | LN | Q |
CD8 | Dako | IR623 (cytoplasmic and membranous) | RU | 20’ min | LN | Q |
CD68 | Dako | IR609 (cytoplasmic) | RU | 20’ min | LN | Q |