Fig. 5

Regulation of macrophages and neutrophils by collagen density and celecoxib. a, b Immunofluorescence images of F4/80⁺ macrophages or Ly6g⁺ neutrophils (magenta), respectively, counterstained with 4',6-diamidino-2-phenylindole (DAPI) (green); ×20 objective; scale bar = 100 um. c, d Index was calculated by dividing positive-stained cells by total numbers of cells. Raw data are depicted. c Quantitation of total F4/80⁺ cells normalized to the total number of cells. F4/80⁺ macrophage numbers are elevated in the stroma of PyMT/Col1a1 tumors and decreased by treatment with celecoxib. d Total number of Ly6g⁺ neutrophils normalized to total number of cells. Ly6g⁺ neutrophil numbers are elevated in the epithelium of PyMT/Col1a1 tumors and decreased by treatment with celecoxib (Clxb); **p < 0.01; n = 5 mice per arm; at least 8 image fields analyzed per 2–3 tumors per animal; mixed linear model. Veh vehicle