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Fig. 4 | Breast Cancer Research

Fig. 4

From: Interferon-induced transmembrane protein 1 (IFITM1) overexpression enhances the aggressive phenotype of SUM149 inflammatory breast cancer cells in a signal transducer and activator of transcription 2 (STAT2)-dependent manner

Fig. 4

Regulation of interferon-induced transmembrane protein 1 (IFITM1) by the interferon signaling pathway in SUM149 cells. a Human interferon α (IFNα) levels were measured in the supernatant and lysates of SUM149 and SUM190 cells after the knockdown of IFNα and interferon regulatory factor 7 (IRF7) using small interfering RNA (siRNA). Measurements of IFNα were done with an enzyme-linked immunosorbent assay (PBL Interferon Source; PBL Assay Science, Piscataway, NJ, USA) according to the manufacturer’s instructions. The data presented are mean ± standard deviation. *P < 0.05 compared with siControl. b IFNα messenger RNA (mRNA) levels in SUM149 and SUM190 IBC cells measured by real-time polymerase chain reaction. *P < 0.05. c Western blot analysis of IFITM1, signal transducer and activator of transcription 1 (STAT1), and STAT2 protein expression following the suppression of IRF7 and IFNα in SUM149 cells. d Left panel: Western blot showing the protein levels of IFITM1, STAT1, and STAT2 after interferon receptor (IFNR)-α/β was neutralized using mouse anti-human IFNR-α/β chain 2 monoclonal antibody (Ab) in SUM149 cells. Right panel: Quantification of IFITM1 protein expression from the Western blots using ImageJ software. *P < 0.05. PUM1 Pumilio RNA-binding family member 1

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Breast Cancer Research

ISSN: 1465-542X

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