Fig. 5

γH2AX response to IR in hMECs. a Representative immunofluorescent images of cells stained for K14 (red), K19 (blue) and γH2AX (green) at 1, 6 and 24 hours post 0 and 5 Gy IR. b Baseline levels of γH2AX in hMEC subpopulations. Mean total γH2AX intensity (log, ± SEM) for each hMEC subpopulation for donor Q685. c Effect of IR on γH2AX intensity and cell cycle profile in two donors with time. Mean total γH2AX intensity (log, ± SEM; left panels) and mean proportion of all cells within G₂/M cell cycle phase (± SEM; right panels) for all cells at 0 Gy (solid line) and 5 Gy (dashed line) over time, and by stratified by cell type (coloured) plotted for 5 Gy treatment only over time, for donors Q685 (top) and Q723 (bottom). Baseline population phenotype heterogeneity is indicated as the frequency of hMEC subpopulations (± SEM) for both donors below the bottom panels. IR ionising radiation