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Fig. 1 | Breast Cancer Research

Fig. 1

From: Epigenetic silencing of CREB3L1 by DNA methylation is associated with high-grade metastatic breast cancers with poor prognosis and is prevalent in triple negative breast cancers

Fig. 1

CREB3L1 gene methylation in breast cancer cell lines, and its inverse correlation with CREB3L1 expression. a A panel of 40 breast cancer cells were analyzed in parallel for CREB3L1 gene methylation (% methylation, blue bars), mRNA (quantitative PCR (qPCR), red bars) and protein expression (quantitative western blot, green bars). Percent methylation (data from Additional file 5: Table S2; left axis labels) were plotted together with the relative CREB3L1 mRNA and protein expression (right axis labels). b The fraction of cell lines with low CREB3L1 protein expression in each breast cancer subtype. c Positive correlation of CREB3L1 protein expression and its mRNA levels in breast cancer cell lines. Spearman correlation (r value) and significance p value. d The fraction of cell lines containing CREB3L1 DNA methylation in each breast cancer subtype. Statistical differences were analyzed by post-hoc pairwise comparison: *p <0.05. e Negative correlation of CREB3L1 mRNA expression and its DNA methylation status in breast cancer cell lines. Spearman correlation (r value) and significance p value. HER2 human epidermal growth factor receptor 2, TNBC triple negative breast cancer

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