Fig. 5

Analysis of Toll-like receptor 4 (TLR4) protein expression in tonsil and breast cancer cells lines. Immunohistochemical analysis using an anti-human TLR4-specific antibody on paraffin-embedded tonsil (a) or cell pellets from the cell lines indicated (b). Arrow indicates the membranous staining. c MDA-MB-231 cells expressing the TLR co-receptors MD2 and CD14 but not ERα left), and MCF-7 cells expressing ERα but not MD2 or CD14 (right) were transfected with GFP-tagged hTLR4 (pUNOI-hTLR4-GFP; Invivogen) for 48 h. Localization was investigated using immunofluorescence microscopy. The green fluorescent protein (GFP)-tagged hTLR4 was expressed in a vesicular pattern in the cytoplasm of MDA-MB-231 cells and evenly in the cytoplasm of MCF-7 cells. Arrow indicates the vesicular pattern. ER+ estrogen receptor-positive, TN triple-negative