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Fig. 2 | Breast Cancer Research

Fig. 2

From: Expression of functional toll like receptor 4 in estrogen receptor/progesterone receptor-negative breast cancer

Fig. 2

Lipolysaccharide (LPS) induced cytokine release in human breast cancer cells in vitro. Release of cytokines by breast cancer cells of estrogen receptor-positive (ER +) origin (a) and triple-negative (TN) origin (b) was analyzed using cytokine bead array (CBA). Unstimulated breast cancer cells of TN origin (b) produce IL-6 and IL-8 at high levels. LPS1 (from S. Typhimurium) and LPS2 (from E. Coli) stimulation for 6 h induced release of IL-8, IL-6 and TNFα from indicated breast cancer cell lines. At least three experiments were performed for each cell line. The relative mRNA expression of IL-6 (c) and IL-8 (d) in unstimulated cells was measured using quantitative real-time PCR (QPCR). At least five experiments were performed for each cell line. Error bars standard error of the mean; ***P <0.001 (analysis of variance)

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Breast Cancer Research

ISSN: 1465-542X

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