Fig. 6

BCL9-KD decreased epithelial cell proliferation in vivo. Immunofluorescent staining of control and BCL9-KD DCIS.COM and SUM225 MIND xenografts stained with the proliferation marker phospho-histone 3 (phosphoH3) (a) and an apoptotic marker cleaved caspase 3 (b) (green), and K5/K19 (red), and 4′,6-diamidino-2-phenylindole (DAPI). White arrows point to the cells with positive staining. The bar graphs represent the number of positive cells per 500 cells; the data represent the mean ± standard error of the mean (n = 4, *p <0.05)