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Fig. 3 | Breast Cancer Research

Fig. 3

From: Expression profiling of in vivo ductal carcinoma in situ progression models identified B cell lymphoma-9 as a molecular driver of breast cancer invasion

Fig. 3

Enhanced BCL9 nuclear expression in DCIS cell line MIND xenografts that progressed to invasive lesions. a, b RT-qPCR of BCL9 in epithelial cell adhesion molecule (EpCAM)-positive epithelial cells sorted from SUM225 (a) and DCIS.COM (b) MIND xenografts at 2, 6, and 10 weeks post-intraductal injection. Bar graphs represent fold change normalized to 2 weeks. Data are mean values ± standard error of the mean (n = 3, *p <0.05). c, d Immunofluorescent (IF) staining of BCL9, keratin (K)5/K19, and 4′,6-diamidino-2-phenylindole (DAPI) in SUM225 (c) and DCIS.COM (d) MIND xenografts at 2, 6, and 10 weeks post-intraductal injection; merged channels are shown in the upper panels and BCL9-only channels are shown in the lower panel. BCL9 was conjugated to Alexa-Fluor 594 (red) and keratin 5/keratin 19 were conjugated to Alexa-Fluor 488 (green). Nuclei were counterstained with DAPI. Scale bars are 50 μm; × 40 objective was used

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Breast Cancer Research

ISSN: 1465-542X

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