Fig. 2
From: Human breast cancer cells educate macrophages toward the M2 activation status
Flow cytometry analysis of CD14+ cells differentiated for 5 days with or without 50 % conditioned media (CM). a-e Percentual variation of median fluorescence intensity (MedFI) of CD14, CD16, CD86, CD200R and CD163 compared to control (CTR), n = 5. f Monocyte colony stimulating factor (M-CSF) protein levels in breast cancer cell line CM (n = 3). g-i Flow cytometry analysis of CD14+ cells differentiated in the presence of IL-10 for 5 days with or without 50 % breast cancer cell line CM. Percent variation of MedFI of CD14, CD16 and CD163 compared to CTR (IL-10 alone). Error bars represent + SD, n = 5 *p <0.05, **p <0.005 (Kruskal-Wallis analysis followed by Dunn’s post hoc test). MB231, MDA-MB231 CM