Fig. 2

Differences in methylation and copy number variation (CNV) between premenopausal (preM) and postmenopausal (postM) estrogen receptor-positive (ER+) tumors: a Heatmap representing significant differences in methylation of 1,738 probes between preM (n = 75) and postM (n = 233) tumors. Red and green indicate higher and lower levels of methylation, respectively. b Heatmap representing significant changes in CNV at gene level between preM and postM ER+ tumors (772 genes). White indicates diploid normal copy, gray indicates single copy deletion (LOH), blue indicates homozygous deletion, yellow indicates low-level copy number amplification, and red indicates high-level copy number amplification. c Peaks of significant amplification (top panels) and deletion (bottom panels) in preM and postM ER+ tumors. Peaks were identified by GISTIC 2.0. The x-axis represents the G score (top) and Q value (bottom). The vertical green line represents the significance cutoff q value = 0.25. Stars indicate significantly different regions of amplifications or deletions comparing preM and postM ER+ tumors. d Mutation distribution in the top five differentially mutated genes (CDH1, GATA3, MLL3/KMT2C, GPS2, PIK3CA). Red, black, and gray indicate truncating, in-frame and other mutations, respectively. Figures were generated with cBio MutationMapper [49, 50]