Fig. 6

An IL-13-STAT6-TP63 signaling axis is associated with improved survival of patients with breast cancer. a MIV cells were stably transduced with shSCR or shSTAT6 lentiviral vectors expressing two different small hairpin RNA oligos (#1 and #2). Western blotting was performed in to measure STAT6 protein levels. The protein levels of α-tubulin were used as loading control. MIV-shSCR and MIV-shSTAT6 cells were treated with 20 ng/ml IL-13 for 24 h, and real-time polymerase chain reaction was performed to measure expression levels of either STAT6 (b) (**P < 0.01, ***P < 0.001) or TP63 (c) (*P < 0.05). d Kaplan-Meier analysis for assessment of DMFS in 1284 patients with breast cancer, based on combined expression of STAT6 and TP63. Survival curves were generated by using the Kaplan-Meier Plotter online tool based on data stratified based on the median. Curves were compared by log-rank test. DMFS distant metastasis-free survival, IL-13 interleukin-13, shSCR scrambled small hairpin RNA, shSTAT6 small hairpin RNA against signal transducer and activator of transcription 6, STAT6, signal transducer and activator of transcription 6, TP63 tumor protein p63