Fig. 5

miR-184 suppresses protein synthesis by negatively regulating AKT/mTORC1 pathway. a Immunoblots of members of the AKT/mTOR pathway in MDA-MB-231 transfected with miR-184 mimics and treated with and without epidermal growth factor (EGF). b Measurement of protein synthesis by using B-Scintillation in MDA-MB-231 transfected with negative control or miR-184 mimics for 24 h, serum starved overnight and treated with labelled H3 leucine. c Measurement of protein synthesis by using B-Scintillation in HEK293E transfected with negative control or miR-184 mimics for 24 h, serum starved overnight and treated with labelled H3 leucine. d Western blot confirming overexpression of AKT2, PRAS40 and GSK3A. e Protein synthesis in cells overexpressing AKT2 (a), PRAS40 (P) and GSK3A or control (empty vector, EV), transfected with miR-184 mimics (184) or controls (Neg)