Fig. 5

Immune mechanisms induced by the preventive vaccine in IL15KO/NeuT and NeuT mice. a Anti-vaccine antibodies. Sera from vaccinated mice were tested against vaccine cells by indirect immunofluorescence followed by cytofluorometric analysis. b Anti-vaccine cell antibody isotypes. Mean ± SEM is shown (eight mice per group). c Yield of splenocyte cultures from 15- and 42-week-old vaccinated mice (IL15KO/NeuT versus NeuT) restimulated by the 6-day co-culture with HER2/neu-expressing syngeneic cells. d IFN-γ production by splenocytes restimulated as in c. Mean ± SEM is shown (three to six mice per group). ^* p <0.05 by the Student’s t test. IFN-γ interferon-gamma, IL15KO/NeuT BALB/c mice transgenic for rat HER2/neu oncogene and knocked out for IL-15, NeuT BALB/c mice transgenic for rat HER2/neu oncogene, SEM standard error of the mean