Figure 5

Functional annotations of the clustering results for triple-negative patients from GSE21653 ( n  = 87). (A) Fuzzy-clustering probability of belonging to clusters, from 0 to 1. (B) Cluster numbering scheme. (C) Clinicopathologic characteristics with significant differences between clusters: age as a continuous colour scale, from 28 years old (pale turquoise) to 85 years old (dark blue); Elston-Ellis (EE) histological grades 1 (pale turquoise), 2 (deep sky blue), and 3 (dark blue); Nottingham prognostic index (NPI) 1 (pale turquoise), 2 (deep sky blue), and 3 (dark blue). (D) Immunohistochemistry results for Ki67: positive (brown) and negative (blue). (E) Molecular subtyping by means of nine gene expression signatures (GES): three single sample predictors (SSPs) - luminal A (dark blue), luminal B (sky blue), HER2-E (purple), basal-like (red), normal breast-like (green) or unclassified (yellow); proliferation (continuous colour scale from minimum (6.01; deep sky blue) to maximum (10.92; red)); TNBCtype (basal-like 1 (red), basal-like 2 (dark red), immunomodulatory (dark green), mesenchymal-like (pink), mesenchymal stem-like (magenta), luminal androgen receptor (blue) and unclassified tumours (yellow)); Teschendorff’s GES (cell cycle (blue), cell cycle and immune response (orange), extracellular matrix (green), immune response (red), steroid hormone response (pink) and unclassified (yellow)); claudin-low (claudin-low (yellow), other (dark blue)); vascular endothelial growth factor (VEGF; continuous colour scale, from minimum (8.12; deep sky blue) to maximum (10.82; red)); and glycolysis (continuous colour scale, from minimum (9.42; deep sky blue) to maximum (13.00; red)). (F) Gene ontology biological process terms enrichment. Missing values are in white.