Figure 5

MMP-8 ablation perturbs the tumor protease web at the protein level. (A) MMP-3 protein expression in tumor tissue at 14 weeks of age as determined by western blotting. Quantification of three biological replicates per genotype, normalized to Hsc70 and compared to PyMT wild-type (WT). Two-tailed unpaired t test, mean ± SEM. (B) Tumor expression of MMP-2 and MMP-9 forms by gelatin zymography at 10 and 14 weeks of age. two-tailed unpaired t test; AU, arbitrary unit; FC, fold change; MMP, matrix metalloproteinase; ns, non-significant; PyMT, Polyoma virus middle T-antigen; SEM, standard error of the mean.