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Figure 5 | Breast Cancer Research

Figure 5

From: FOXF2 deficiency promotes epithelial-mesenchymal transition and metastasis of basal-like breast cancer

Figure 5

FOXF2 binds to TWIST1 promoter and suppresses TWIST1 transcription. (A) The putative binding site of FOXF2 on the TWIST1 promoter region. (B) The binding of FOXF2 on the TWIST1 promoter region containing the putative binding site in MCF-10A cells was tested by a ChIP assay. (C) Transcriptional activity of TWIST1 promoter in the indicated cells was assessed by a dual-luciferase reporter assay. (D) TWIST1 protein expression in the indicated cells was detected by immunoblot. (E) TWIST1 protein expression in the indicated cells was detected by immunofluorescence staining. Three independent experiments were performed in triplicate. The data were expressed as means ± SD. * P <0.05; ** P <0.01. ChIP, chromatin immunoprecipitation; SD, standard deviation.

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Breast Cancer Research

ISSN: 1465-542X

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