Clinically relevant dose regimen of L-NMMA in orthotopic mouse models of triple-negative breast cancer. (A) Mean systolic pressure of mice (n = 5) giving one cycle of the dose rate proposed in this study. (B) Mean systolic pressure of mice 30 minutes and 24 hours after the last injection of one cycle treatment (n = 5). (C) Tumor volume of MDA-MB-231 xenografts (n = 10 per group) treated with vehicle, docetaxel, and combination (docetaxel + amlodipine + L-NMMA). (D) Kaplan-Meier survival curve of vehicle-, chemotherapy-, and combo-treated MDA-MB-231 xenograft-bearing mice. (E) Tumor volume of SUM159 xenografts (n = 10 per group) treated with vehicle, docetaxel, and combination. (F) Effects of amlodipine on tumor volume in MDA-MB-231 xenografts (n = 5 per group). Data are presented as mean ± standard error of the mean. *P <0.05, **P <0.01, ***P <0.001, ****P <0.0001. L-NMMA, NG-monomethyl-L-arginine; ns, not significant.