Figure 3

Molecular profiling of IR and IGF1R signaling in insulin analogue derived mammary gland tumors. Tumor protein levels of critical mammary gland tumor related receptors (IR, IGF1R, ER, EGFR, Her2) and downstream signaling pathways (Erk, phospho-Erk, Akt, phospho-Akt) as well as epithelial differentiation markers (N-cadherin and E-cadherin) were determined by quantitative Western blotting of all primary mammary gland tumors (n = 148). A) A small subset of the Western blot data (n = 36) is shown which are representative for the quality of the blots. B) The quantitative IR, IGF1R, p-Akt and p-Erk levels of all primary EMT tumors are presented in dot-plots (n = 148). C) The IR versus IGF1R protein levels are plotted, five clusters are defined. D) The mean tumor latency time has been determined of each cluster. Error bars represent SEM, ns = P >0.05, * = P <0.05, ** = P <0.01, *** = P <0.001. EGFR, epidermal growth factor receptor; EMT, epithelial to mesenchymal transition; ER, estrogen receptor; IGF1R, insulin-like growth factor 1 receptor; IR, insulin receptor; SEM, standard error of the mean.