Figure 5

Elevated mammary tumor cell BST-2 regulates cancer cell adhesion. PKH67Green-labeled BST-2-expressing shControl and BST-2-suppressed sh137 and sh413 E0771 cells were allowed to adhere to murine embryonic fibroblasts (MEFs). (A) Representative confocal images of adhered cells and (B) Image J quantification of PKH67Green + cells per field (n = 5) are shown. Images were taken at 20X. (C) Analysis of cancer cell adhesion to MEF by quantification of luciferase bioluminescence shControl and sh413 cells. Luciferase assay quantified expression represented as relative light units (RLU) and images (inset) were taken with the IVIS three-dimensional optical imaging system and analyzed with Living Image Software. (D) Representative images and (E) quantification of shControl and sh413 4T1 cells adhered to collagen-coated plates. (F) Representative images and (G) quantification of shControl and sh413 4T1 cells adhered to fibronectin-coated plates. Images were taken at 10X. Experiments were performed multiple times with similar results. Error bars represent standard deviations and significance was taken at P <0.05^*, <0.01^**. ns = not significant.